NANOEMULSION-BASED MOUTHWASH OF ETHYL ACETATE FRACTION OF SERAI WANGI STALK: FORMULATION, CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY TEST

Serai wangi or citronella (Cymbopogon nardus L.) has long been used in Indonesia as a component of traditional medicine. A recent study aimed to determine the antibacterial activity most active fraction Citronella stalk extract and formulate it into nanoemulsion mouthwash. Fractionation ethanolic was carried out using liquid-liquid extraction, found that ethyl acetate (EAF) active. This then varied at concentrations 25%, 12.5%, 6.25%, 3.125%, 1.5625%, 0.78125% for test disc diffusion method. To potency microbial activity, minimum inhibitory concentration (MIC) bactericidal (MBC) were evaluated. Subsequently, formulated characterized by measuring transmittance percentage, droplet size, polydispersity index (pdI) particle size analyzer. The results showed 25% obtained an inhibition zone diameter 10.67 mm. MIC MBC values ??were 6.25%. In addition, characterization formulae exhibited pdI follow 101.6 nm 0.681 (Formula I); 84.1 0.609 II); 108.3 0.527 III). Keywords: stalk; fractionation; Streptococcus mutans; nano-emulsion; mouth wash